Sar1B drives tubule fission in a GTP-dependent manner. Effect of wild-type Sar1B on tubule density in the presence of 5 µM GDP, 5 µM GTP, and 5 µM GMP-PNP. In these experiments, 50 nM Sar1B was titrated onto an SLB containing approximately 100 pre-formed tubules in each field of view sampled. *:P
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