Nelfinavir analogs inhibit proliferation and induce apoptosis in CRPC.(A) Nelfinavir analogs inhibit CRPC proliferation. DIMSCAN assay was performed in nelfinavir- or analog #6, 7, 8, 31, 39-treated DU145 and PC-3 cells. 1000 cells were seeded in a 96-well plate for overnight incubation followed by treatment with DMSO, nelfinavir or its analogs (10 μM) for 3 days. Each experiment was performed in triplicate. Results are presented as mean ± SD. *p < 0.01, +p < 0.05 compared to DMSO control. (B) Nelfinavir analogs induce CRPC apoptosis. DU145 and PC-3 cells were treated with DMSO or 10 μM of nelfinavir or analog #6, 7, 8, 31, 39. After 24 hr, cells were harvested and stained with Annexin V-FITC for detection of apoptosis. Image represents three repeated experiments.
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