20 Multiplexed Transcription Factor Assays Modulation of TF activity in human hepatoma HepG2 cells Multiplexed reporter gene assay –cis 52 assays (response element driving reporter) –trans 29 assays (GAL4-NR_LBD driving reporter) ligand detection IC50 for cytotoxicity measured first in HepG2 High concentration either 100  M or 1/3 calculated IC50 for cytotoxicity Seven concentrations, 3-fold serial dilutions, 24 hr exposure Cells harvested, RNA isolated, processed for reporter gene quantitation LEL provided in data set
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