Creating transgenic cotton plants by Agrobactrium-mediated genetic transformation. (A) Callus induction on selective media containing kanamycin. (B) Embryogenic callus emerged from non-embryogenic callus. (C) Somatic embryos and young plantlets developed from embryogenic callus. (D), (E)and(F) Regenerated putative transgenic plants cultured in rooting media, water and soil. (G)Identification the positive and negative plants of T1 progeny on selective media containing kanamycin. (H)Positive T1 transgenic progeny cultivated in greenhouse.
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