One µg of IL-12 as separated from histidine and rHSA on a BioResolve RP mAb Polyphenyl Column. The flow rate was 0.3 mL/min. (B) Chromatogram overlays at various injected masses of IL-12 as separated from rHSA on the BioResolve RP mAb Polyphenyl Column. The flow rate was 0.3 mL/min. (C) IL-12 peak area calibration data (0.01–2 µg) were constructed from Figigure 1B information. Two linear ranges (0.01–0.1 µg and 0.1–2 µg)  were identified that resulted in a calculated mass load error of less than 8%. The flow rate  was 0.3 mL/min.
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