Effect of metformin, cisplatin and metformin/cisplatin on apoptosis in MKN-45 cells. A) Hoechst 33342 stains the condensed chromatin in apoptotic cells more brightly than the chromatin in normal cells and Propidium Iodide (PI) is only permeate to dead cells (FL2: PI, FL6: Hoechst 33342). As shown in figure, there is significant change in the apoptosis rate of metformin, cisplatin and metformin/cisplatin treated MKN-45 cells compared with the control. B) Quantified values of apoptosis in MKN-45 cell line treated with metformin, cisplatin and metformin/cisplatin. As shown in this figure, the cisplatin-induced apoptosis at concentration of 5 μM is significantly higher than the combination of metformin 10 /cisplatin 5. Statistically different values of *P<0.05 and **P<0.01 are determined compared with the control
{}