Detection of <t>TH2B</t> protein using Western blotting and regression models showing variation in the intensity of TH2B in sperm as related to fertility scores in Holstein bulls. a MM stands for molecular markers; LF1–5 and HF1–5 refer to samples from low vs. high fertility bulls, respectively. The same amounts of nuclear proteins were loaded into each lane. As TH2B is a nuclear protein and the expression levels of our internal control (Lamin B1) were not stable, another protein band (~25 KDa) served as the internal control. b The regression line was determined using the model predicted intensity values for each fertility score using the mixed effects model. A scatter plot of unadjusted data points was superimposed on the regression line plot. Regression equations are shown for high fertility ( y = −22,315 × + 89,049) and low fertility ( y = 9603 × + 50,993) bulls
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