Figure 1. Evidence for Sertoli cell proliferation in men. Confocal immunofluorescence of proliferation markers in testis tissue from normal (A and E) and gonadotropin suppressed men [2 wk, B; 12 wk, C and D (enlarged portion of C) and F and G (enlarged portion of panel F)], in testis with carcinoma in situ (H) and in a tubule adjacent to a seminoma (I). Sections were probed with a combination of either GATA4 (green, in the nuclei of Sertoli cells) within the seminiferous epithelium (asterisks), and PCNA antibodies (red, staining cells with proliferation capacity - triangles) (A–D, H–I), or GATA4 (red) and Ki67 (green, staining proliferating cells) (E–G). Colocalization of GATA4 and PCNA or GATA4 and Ki67 in Sertoli cell nuclei (indicating a proliferative state, arrows) is shown by a yellow color. Figure 1E–G includes a transmitted light channel to illustrate histological detail. Inserts are negative controls. (Bar = 50 µm).
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