TRPA1-dependent 15d-PGJ2-mediated inhibition of AITC in vitro and mechanical hypersensivity in vivo. (A) In the presence of a specific TRPA1 antagonist, HC-030031 (100 μM), 100 μM 15d-PGJ2 did not block 50 μM AITC responses. (B) We also examined the reversibility of AITC-response inhibition in DRG neurons by 15d-PGJ2. 100 μM 15d-PGJ2 inhibited subsequent responses to 50 μM AITC for up to 16 min with continuous washout. Traces depict representative cells from 1 or more experiments in which >100 neurons were analyzed per experimental condition. (C) 15d-PGJ2 (10 μL ipl.) attenuates CFA-induced mechanical hypersensitivity in WT but not TRPA1 −/− mice. Vehicle or 15 mM 15d-PGJ2 was injected into the hindpaw of TRPA1 or WT littermates (n = 8 per group) 1 h prior to von Frey measurements. **p < 0.01 in comparing WT mice injected with 15d-PGJ2 vs. WT mice injected with vehicle. #p < 0.05, ##p < 0.01 in comparing WT mice injected with 15d-PGJ2 vs. TRPA1 KO mice injected with 15d-PGJ2. Values expressed as mean ± SEM. Data were analyzed using RMANOVA with Bonferonni post-hoc comparisons.
{}