MYC3 and MYC4 regulate expression of JA-responsive genes. (A, B) qRT-PCR expression analysis of JAZ genes and wound-responsive genes in 10-d-old wild-type and MYC overexpression seedlings without JA treatment. Wild-type samples served as a calibrator for the calculation of relative expression levels (arbitrarily set to one). (C) Relative transcript levels of the PDF1.2 gene in 10-d-old wild-type and overexpression transgenic seedlings with or without 10 μM JA treatment (incubated for 6 h). Wild-type samples without JA treatment served as a calibrator, and relative expression was determined from replicate measurements in two independent biological replicates. Data are mean ±SE.
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