Effect of acute ACTH treatment on BrdU-pulse-chase-labelled cells in the adult mouse adrenal cortex.Adrenal sections from female F1 mice treated acutely with ACTH 6 weeks after 1-week BrdU infusion, immunostained for (A,B) BrdU, (C,D) Ki67 and (E,F) BrdU/Ki67. Mice (n =6/group) were injected with saline vehicle (A,C,E) or ACTH (B,D,F), as described in the Materials and Methods section. Ki67-positive cells are indicated by brown nuclear staining while BrdU-labelled cells are identified by blue nuclear staining. Typical examples of BrdU/Ki67 dual-labelled cells are shown by arrows in F. Sections C and D were lightly counterstained with haematoxylin but sections A,B,E & F were mounted without counterstaining. Scale = 100 µm.
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