Figure 5. Consensus sequences of the -35 region.  (A) Published -35 consensus based on 263 naturally occurring  E. coli  promoters (Harley & Reynolds, 1987); (B) Preliminary pClone -35 consensus was determined from the the top 12 mutant promoters with >50% efficiency compared to Ptac; (C) pClone -35 consensus was determined from sequences found in all 81 selected promoters. using a weighted consensus method.
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