Effect of MCD on BDNF modulation of synaptic fatigue at hippocampal CA1 synapses. Neonatal hippocampal slices were pretreated with MCD (2 mM) for 30 min before treatment with BDNF (2 nM) for 1–2 h. A train of HFS (100 Hz, 1 s) was applied to Shaffer collaterals and field EPSPs were recorded from stratum radiatum. The slopes of EPSPs during the entire recording were normalized to the first EPSP slope in each recording. (A) An example of EPSPs elicited by HFS recorded from a BDNF-treated slice. (B) Effect of MCD on synaptic depression induced by HFS. Normalized EPSP slopes in each condition were averaged and plotted against the number of stimulus during HFS. (C) Summary of the MCD effect on the rate of synaptic depression. The plot for each recording was fitted with a single exponential curve, and rate constant (τ) for each condition was averaged and presented. *Indicates significantly higher than all other groups; ANOVA followed by post hoc tests; P < 0.001. The number associated with each column represents the number of slices used for each condition.
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