Ovaries produce 20E after blood meal in A. gambiae . ( A ) RT-PCR analysis of CYP306A1 , CYP302A1 , CYP315A1 , and CYP314A1 expression pattern in ovaries throughout the first gonotrophic cycle (NBF, ovaries from non-blood-fed females; 6, 18, 24, and 48 h, ovaries at different times PBM) and in different body parts of 24 h PBM blood-fed females (GM, gut and Malpighian tubules; Ca, carcass). ( B ) In vitro ecdysteroid secretion by ovaries during the first gonotrophic cycle. (NBF, ovaries from non-blood-fed females; 6, 18, 24, and 48 h, ovaries from blood-fed females at different times PBM.) ( C ) HPLC-EIA analysis of ecdysteroids secreted by ovaries of blood-fed females (24 h PBM). Results are expressed as E (solid line) and 20E (dotted line) equivalents. ( D ) In vitro ecdysteroid secretion by different body parts of blood-fed females (24 h PBM). (He, head; Th, thorax; Gu and Mt, gut and Malpighian tubules; Ov, ovaries; Ca, carcass.)
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