Knockdown of Irm inhibits myogenic differentiation. a Relative expression levels of Irm in C2C12 cells expressing si-scramble or si-Irm, as detected by qRT-PCR. b The differentiation of Irm -knockdown C2C12 cells was detected by staining for MHC at 96 h in DM. Fusion index was calculated. Scale bars, 50 mm. c, d The mRNA levels of myogenin ( c ) and MHC ( d ) were detected by qRT-PCR in Irm -knockdown C2C12 cells at 0, 1, 3, and 5 days in DM. e, f The protein levels of myogenin ( e ) and MHC ( f ) were detected by western blotting in Irm -knockdown C2C12 cells at 0, 1, 3, and 5 days in DM. Myogenin protein levels were normalized to GAPDH protein levels. Data shown represent the mean ± SEM of three independent experiments. * P
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