Heterogeneity of adenosine sensitivity is also apparent in rat somatosensory cortex. Heterogeneity of adenosine sensitivity is also apparent in rat somatosensory cortex. (A) Morphological reconstruction of the soma and dendrites from pyramidal neurons in layers 2 and 5 of the somatosensory cortex. Inset at left bottom shows an example staining with layer borders indicated as dotted lines. (B) Left, average response of the RMP of excitatory neurons in layers 2 and 5 during bath application of 100 μM adenosine (start at arrow). Right, average adenosine-induced hyperpolarization of layer 2 pyramidal neurons (n = 5) and layer 5 pyramidal neurons (n = 15). (C) Example trace of the RMP during application of 100 μM adenosine, followed by coapplication of 5 μM A1R antagonist CPT. Right, average responses during control (con) and 100 μM adenosine (ado) conditions, and during coapplication of 1–5 μM CPT (n = 5). (D) Adenosine-induced hyperpolarization of the RMP for layer 5 pyramidal neuron subtypes (slender tufted, n = 6; broad tufted, n = 3; untufted, n = 6). Sublaminar location of neurons is indicated for layer 5A (upward triangles) or 5B (downward filled triangles). *P < 0.05, **P < 0.01.
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