Detection of Human and Mouse GAPDH by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg) and mouse NIH3T3 (M; 50 mcg). Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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