Percentage of Th1, Th2, and Th0 cells in nonpregnant women, normal pregnant women, and preeclamptic patients. Peripheral blood mononuclear cells were stained with Peridinin chlorophyll protein (PerCP)-conjugated CD4 monoclonal antibody, <t>fluorescein-isothiocyanate</t> <t>(FITC)-conjugated</t> anti IFN-γ monoclonal antibody and PE-conjugated anti-interleukin (IL)-4 monoclonal antibody, as described in Materials and Methods. A gate was set on the CD4 + lymphocytes by characteristic forward and side scatter parameters (R1 and R2). The FITC (anti-interferon (IFN)-γ) and PE (anti-IL-4) fluorescence data were analysed. Isotype muched fluorochrome-conjugated IgG1 and IgG2a were used as control. The horizontal bar shows FITC fluorescence and the vertical bar shows PE fluorescence. Fluorescence intensity is determined on a logarithmic scale.
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