Both proliferation and differentiation of Ccn2 -deficient (KO) myoblasts is impaired in vivo . ( A ) Double immunoreactivity for PCNA and MyoD was detected in the sections of the skeletal muscle from E18.5 WT and KO littermates by immunofluorescence analysis, and the bottom graph shows the means and standard deviations of the number of double positive cells from three different views. ( B ) Immunoreactivity for desmin was detected in the sections from WT and KO skeletal muscle tissues, and the bottom graph shows the means and standard deviations of areas of muscle fibers stained with anti-desmin antibody. * P
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