LMWPTP influences cell adhesion and cell morphology(A) CRC cell adhesion was determined by MTT assay of adherent cells after indicated time points, with fibronectin (FN) coating serving as control. CACO-2 LMWPTP knockdown cells adhere less than control cells (*P < 0.05; **P < 0.01). (B, C) Confocal microscopy of Phalloidin-rhodamine stained cells was employed to examine cell morphology. Immunofluorescence reveals a more elongated morphology in HCT116 LMWPTP knockdown cells as compared to control (B). To quantify the changes in morphology, the ratio between the length and width of the cells was calculated, with a higher ratio indicating a more elongated shape. Non-target control cells have a significantly lower ratio compared to LMWPTP knockdown cells. (1.1 ± 0.02 vs 1.7 ± 0.18, n = 26; P < 0.0001) (C).
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