Effect of BAK treatment on Cx43 and ZO-1 expression. The significant decrease of Cx43 protein was induced by topical application of BAK at the concentration of 0.05% and 0.1% (C, D). In comparison, there was no significant decrease of Cx43 and ZO-1 mRNA in BAK treated group (A, B). Similarly, the expression of ZO-1 protein was consistent with mRNA level of ZO-1. Quantitative analysis of Cx43 mRNA and protein in corneal endothelium was shown in (B) and (D) respectively. Data are means ± SE from three independent experiments. *P value
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