Addition of Galgp restores binding of M. avium by CD43−/− Mφ. (A) M. avium were incubated for varying times with CD43−/− Mφ in the absence (○) and presence (⋄) of 100 μg/ml Galgp. Bacteria/cell ratio was 2:1. The Mφ were harvested and extensively washed, and adherent bacteria were quantified. The binding of M. avium to CD43+/+ Mφ in the absence of Galgp is shown for comparison (•, dashed lines). (B) M. avium at varying bacteria/cell ratios as indicated were incubated with CD43−/− Mφ for 4 h in the absence (○) and presence (⋄) of 100 μg/ml Galgp. Shown are mean values ± SEM for three mice of each group. The number of mycobacteria associated with CD43−/− Mφ in the presence and absence of 100 μg/ml Galgp was significantly different in all conditions (P < 0.0001). Comparable effects of Galgp addition were observed in two additional experiments. Galgp at 200 μg/ml produced similar results (data not shown).
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