MLN4924 treatment induces cleavage of apoptotic proteins A375 melanoma cells were treated for 72 hours with 0 nM, 1200 nM or 600 nM MLN4924. Cells were lysed and protein was harvested and subjected to immunoblot analysis for total or cleaved caspase-3, caspase-7, caspase-9 or PARP. β-actin was utilized as a loading control.
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