The nucleotide specificity of EGFP-SRm160 mobility in digitonin-permeabilized cells. HeLa cells stably expressing EGFP-SRm160 were permeabilized with digitonin. Before photobleaching, either buffer alone, 5 mM ADP, ATP, GTP, or AMP-PNP, or a combination of ATP and GTP (5 mM each) were added. Each point represents the mean for the number of photobleached cells indicated in parentheses to the right of the curve.
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