Basic Cloning Process Plasmid is cut open with a restriction enzyme that leaves an overhang: a sticky end.
{"Image DocumentName": "Basic Cloning ProcessPlasmid is cut open with a restriction enzyme that leaves an overhang: a sticky end.", "Image ImageDescription": "Foreign DNA is cut with the same enzyme. The two DNAs are mixed. The sticky ends anneal together, and DNA ligase joins them into one recombinant molecule. The recombinant plasmids are transformed into E. coli using heat plus calcium chloride. Cells carrying the plasmid are selected by adding an antibiotic: the plasmid carries a gene for antibiotic resistance.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}