Determination of levels of TNF-α and <t>IL-6</t> in small intestines of AG129 mice after inoculation of ICs. Groups of AG129 mice ( n = 3) were challenged with a sub-lethal dose of DENV-2 S221 (V) or 100% in vitro -neutralized ICs of DENV-2 S221 with mAb 4G2 (V+4G2 ICs), anti-mE1E2 bv VLP antiserum (V+α-mE1E2 bv VLP ICs), anti-pmE1+E2 VLP antiserum (V+ α-pmE1+E2 VLP ICs), anti-mE3E4 bv VLP antiserum (V+α-mE3E4 bv VLP ICs), or anti-DENV-2 S221 antiserum (V+ α-DENV-2 S221 ICs), in parallel with the inoculations described in Figure 6B . One group was included as negative control (NC) which did not receive any treatment. Three days post-inoculation mice were euthanized, small intestines dissected out after perfusion and homogenized. Clarified homogenates were used for the determination of TNF-α (A) and IL-6 (B) using commercial <t>ELISA</t> kits, with purified recombinant murine TNF-α and IL-6 as references. Data shown are the mean values with the bars denoting standard deviation ( n = 3).
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