Reactive oxygen species (ROS) and nitric oxide (NO) production as well as cell death in zebrafish. The zebrafish embryos were exposed to lipopolysaccharide (LPS) (10 μg mL-1) and treated with polyphenol-rich fraction (PRF; 25, 50, and 100 μg mL-1). (A) ROS level. (B) NO level. (C) Cell death. Assessments were measured by image analysis and fluorescence microscope. The fluorescence intensity of individual zebrafish was quantified using image J program. Experiments were performed in triplicate and the data are expressed as the mean ± standard error. a-dValues with different alphabets are significantly different p < 0.05 as analyzed by Duncan's multiple range test.
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