TCR Vβ repertoires of vaccine-elicited p11C, p41A, or p68A epitope-specific CD8 + T-lymphocyte populations do not change following infection with a nonpathogenic SHIV. PBMC from monkeys (Mm) 90-98 (A), 128-97 (B), and 196-97 (C) were isolated 4 (wk4 pi) and 19 (wk19 pi) weeks following SHIV-89.6 infection. The lymphocytes were cultured in vitro with p11C, p41A, or p68A peptide; stained with tetramers; and sorted. cDNA synthesized from the RNA extracted from these tetramer-binding CD8 + T-lymphocyte populations was used to evaluate Vβ repertoires.
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