Effect of chloroquine and rapamycin on LC3-I and LC3-II expression. Human spermatozoa were incubated for 2 hours at 37 °C in presence or absence of chloroquine ( a ) and rapamycin ( b ). Proteins were extracted and resolved by SDS_PAGE. Immunoblotting was performed with anti-LC3 antibody (described in materials and methods section). Results are expressed as increase of LC3-II/LC3-I ratio respect to control samples (containing only vehicle). Columns with asterisk indicate significant differences (P
{"Image ImageWidth": "2700", "Image ImageLength": "1568", "Image BitsPerSample": "8", "Image Compression": "LZW", "Image PhotometricInterpretation": "1", "Image Orientation": "Horizontal (normal)", "Image SamplesPerPixel": "1", "Image XResolution": "300", "Image YResolution": "300", "Image ResolutionUnit": "Pixels/Inch", "Image Software": "Adobe Photoshop CS4 Windows", "Image DateTime": "2016:09:02 18:02:59", "Image ExifOffset": "236", "Thumbnail Compression": "JPEG (old-style)", "Thumbnail XResolution": "300", "Thumbnail YResolution": "300", "Thumbnail ResolutionUnit": "Pixels/Inch", "Thumbnail JPEGInterchangeFormat": "374", "Thumbnail JPEGInterchangeFormatLength": "4532", "EXIF ColorSpace": "Uncalibrated", "EXIF ExifImageWidth": "1395", "EXIF ExifImageLength": "810"}