Effects of one freeze–thaw cycle on the quality of HMM-propelled actin filament motility. Data from five different experiments. The control velocity was 5.97 ± 0.63 μm/s, and the control fraction of motile filaments was 0.74 ± 0.04. The duration of the freezing period varied between 4 and 30 days (average 16.00 days) without an appreciable difference in the effect on motility over this period. Error bars: SEM.
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