Immunohistochemical staining of IGFBP-2 in human adipocytes and MMP-2 in human breast tumors.4A. Sections of metastatic and non-metastatic breast tumors and surrounding adipocytes were stained by hematoxylin and eosin (magnification 100 ×). Peritumoral adipocytes from infiltrant metastatic mammary tumors appear smaller and more spindle-shaped. 4B. Representative microphotographs show IGFBP-2 in human adipocytes around metastatic and non-metastatic breast tumors. Human adipocytes around metastatic breast tumors had higher levels of IGFBP-2 than did those around non-metastatic primary tumors. Arrowheads indicate IGFBP-2 positive cells (magnification 100 ×). 4C. Representative microphotographs show MMP-2 in metastatic and non-metastatic human breast tumors. Metastatic human breast tumors had higher levels of MMP-2 than did non-metastatic tumor tissue. Ellipse indicates MMP-2 positive cells at the edge of tumor (magnification 100 ×). The negative control was obtained by omitting the primary antibody.
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