Selective expression of E1A in MUC1-positive cells infected with Ad.DF3-E1. ( a ) The indicated cells were incubated with either mAb DF3 (open area) or an isotype-identical control Ab (shaded area) and then subjected to flow cytometric analysis. ( b ) Cells were infected with Ad.DF3-E1, Ad.CMV–β-gal, or wild-type Ad5. Lysates from control (noninfected) and infected cells were subjected to immunoblot analysis with anti-E1A Ab. ( c ) Lysates from Ad.DF3-E1–infected cells were subjected to immunoblot analysis with anti-E1B.
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