Separation of the phenol mixture with elution gradient using as mobile phase: Phase A: acetonitrile-water (pH 4.5) (15:85) v / v, and phase B: acetonitrile-water (pH 4.5) (70:30 ). Acetic acid-sodium acetate buffer. The separation time is approximately 40 minutes. Flow 1 mL / min, UV detector. Sensitivity 0.05 AUFS. The order of elution of the analytes is: 1) 4,6-dinitro-2-methylphenol, 2) 2,4-dimethylphenol, 3) 4-chloro-3-methylphenol, 4) 2,4-dichlorophenol, 5) 2 , 4,6-trichlorophenol and 6) pentachlorophenol.
{}