The CD8 + T cell response to Rv0634A 19-29 is restricted by HLA-E. A) A549 cells were pulsed with the Rv0634 19-29 peptide and used as antigen presenting cells in an ELISPOT assay with IFN-γ production by CD8 + T cells from mismatched donors as a readout. B-D) D481 CFSE-labeled CD8+ T cells were isolated and cultured with Rv0634A 19-29 peptide-pulsed autologous macrophages and DC. CFSE-dim cells were sorted and stained with the Rv0634A 19-29 HLA-E tetramer and antibodies against CD3 and CD8. As a control, the D481 line was also stained with a non-HLA-E tetramer (C), or with anti-NKG2A (D) do demonstrate that the tetramer staining is specific. Whole PBMC were stained with the anti-NKG2A antibody as a positive control. E) The mismatched A549 cell line was pulsed with the Rv0634A 19-29 peptide and used as antigen presenting cells with D481 CD8 + T cell line.
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