Figure 1: Effects of age on KCl- and acetylcholine-induced contraction. The KCl (70 mM)-induced maximal contraction in 12- and 24-month mice were not significantly different (<i>n</i> = 11-8, <i>P</i> > 0.05, a). A representative tracing shows acetylcholine (acetylcholine, 0.001–10 μM)-induced contraction in 12-month (upper panel, b), and 24-month (lower panel, b) mice, in urothelium intact urinary bladder in the absence of active muscle tone. This constriction was not different between 12- and 24-month bladder strips (<i>n</i> = 11 ~ 6, <i>P</i> > 0.05, c). Values are mean ± standard error mean, <i>n</i> = number of experiments. W: Wash
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