Agarose yield gel results
{"Image DocumentName": "Agarose yield gel results", "Image ImageDescription": "Degraded DNA sample. D5S818. D13S317. D7S820. D16S539. CSF1PO. Penta D. Agarose yield gel results. Smear of degraded DNA fragments. High molecular weight DNA in a tight band. (A) (B) Good quality DNA. Degraded DNA. Figure 7.1 Impact of degraded DNA on (A) agarose yield gel results and (B) STR typing. (A) Degraded DNA is broken up into small pieces that appear as a smear on a scanned yield gel compared to good quality DNA possessing intact high molecular weight DNA. (B) Signal strength is generally lost with larger size PCR products when STR typing is performed on degraded DNA, such as is shown from the green dye-labeled loci in the PowerPlex 16 kit. Thus, 180 bp D13S317 PCR products have a higher signal than 400 bp Penta D amplicons because more DNA molecules are intact in the 200 bp versus the 400 bp size range. Figure 7.1, J.M. Butler (2005) Forensic DNA Typing, 2nd Edition \u00a9 2005 Elsevier Academic Press.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}