Assessments of fibrinolytic activity in BAL samples. Generation of fibrinolytic activity by BAL fluids in the presence of human plasminogen. Box plots: relative fibrinolytic activity (FA), produced by BAL fluids (Gr1: 5 saline-controls, GR1 TRX: 11 scuPA and tPA treated animals; Gr2: 6 saline controls, 5 and 7 animals treated with 4 and 8 mg of nebulized scuPA respectively, and 5 animals treated with 4 mg nebulized tPA) in the presence of 100 nM human Glu-plasminogen. Data are presented as box plots (showing interquartile ranges). No statistically significant differences were noted in the median value of FA between the groups. Inset: enzymographic analyses of BAL fluids from animals with ISIALI. BALs were collected at 48 h after initiation of the treatment at the time of euthanasia. Arrows to the right of the panels indicate positions of: tPA/PAI-1 inhibitory complexes (110 kDa) (I), uPA/PAI-1 inhibitory complexes (100 kDa) (II), tPA (63 kDa) (III), uPA (50 kDa) (IV). To illustrate the range of activity in the treatment groups, samples the range of ones with low levels of PA and relatively higher PA levels from sheep included in Group 2. Lanes: recombinant human uPA (0.5 ng) and tPA (0.1 ng) standards are shown in lanes 1 and 2, respectively. 3: saline treated animal, 4 and 5—8 mg scuPA-treated animals, 6 and 7—4 mg tPA-treated animals. The data are representative of the findings in all similarly treated Group 2 animals
{}