Western blot analysis of Phospho-GATA3 (S308) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />  Positive control: <br />  Lane 1: human skin tissue lysate<br />  Lane 2: Jurkat cell lysate
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