Role of methylation of S-HDAg in HDV RNA replication. The HDV genomic (A) or antigenomic (B) RNA was transfected together with S-HDAg mRNA, in vitro-methylated S-HDAg, or unmethylated S-HDAg into Huh7 cells. At 3 days posttransfection, total cellular RNAs were extracted, and antigenomic RNA (A) or genomic RNA (B) was detected by Northern blotting.
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