Effects of A-domain  mutants on CR3 surface expression and iC3b binding.  (A) Histograms (mean ±  SEM, n = 3) showing the relative binding of anti-CD11b  mAb OKM10 to COS cells  expressing mutant CR3. (B)  Histograms (mean ± SEM,  n = 3) showing the relative  binding of iC3b to COS cells  expressing CR3 mutants. (Inset) Radioautograph of a  Western blot showing comparable amounts of the  CD11b subunit in anti-CD18  immunoprecipitates from  COS cells expressing WT or  mutant CR3. No CD11b was  seen in anti-CD18 immunoprecipitates from COS cells  transfected with CD18  cDNA alone.
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