RNase protection assays indicate the lack of α2-isoform in guinea pig ventricular myocytes that lacked the stimulation of IP. mRNA for the α1-, α2- and α3-isoforms of the Na/K pump are abundantly expressed in guinea pig brain. In contrast, the α1-isoform is the dominant transcript in guinea pig ventricle. The α2-isoform is present at very low levels, just 6 ± 4% (SD) of the total Na/K pump mRNA based on quantification from three different samples (compared with 18% in normal samples). No α3-isoform mRNA was detected from any guinea pig heart sample.
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