siRNA against human genes inhibits T. gondii growth. HeLa cells were transfected with siRNA pools to the indicated gene for 24 hours, then infected with 2.5×10 4 mCherry-expressing parasites. Fluorescent measurements were taken at 24, 48, and 72 hours post infection. (A) Replication of T. gondii as measured by fluorescence in HeLa cells transfected with DDX48 siRNA (DDX48, squares), AllStars Negative Control siRNA (negative, circles), or AllStars Hs Cell Death Control siRNA (death, triangles). (B) Replication of T. gondii graphed as the percent growth of wells treated with AllStars Negative Control siRNA (% negative siRNA) at 24 (black), 48 (red), and 72 (blue) hours post infection. Asterisks indicate a significant (p
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