- DHA triggered priming of inflammasome signaling pathway. Breast cancer MDA-MB-231 cells were stimulated with 50 or 100μM of DHA or AA ( A ) for 18 h and NFκB translocation towards to nucleus was analyzed by confocal microscopy (NF-κB in red and nuclei in blue) compared to unstimulated cells (UNS). MDA-MB-231 cells were also stimulated with 50, 100 or 200μM of DHA ( B ) for 6 h and expression of pro-caspase-1, pro-IL-1β and adaptor protein ASC was analyzed by western blotting and bands densitometry was performed using Image J software ( C ).
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