Emodin affected the percentage of viable cells and DNA fragmentation in WEHI-3 cells. Cells (1 × 104 cells/well; 96-well plates) were plated in RPMI 1640 medium + 10% fetal bovine serum (FBS) with 0, 25, 50, 100, and 150 μM of emodin for 24 and 48 h. The cells were collected by centrifugation and the viable cells were determined by using the MTT assay (a). Cells were treated with 0, 50, and 100 μM of emodin for 24 h, and then DNA was isolated for DNA gel electrophoresis (b) as described in Section 2. Columns, mean of three determinations; bars, SD. a, P < .05 shows significantly different when compared with DMSO-treated control; b, c, and d, P < .05 indicates significantly different compared with 25, 50, and 100 μM emodin-treated groups, respectively (one-way ANOVA followed by Bonferroni's test for multiple comparisons).
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