Effect of recombinant EMMPRIN on the secretion of MMP-9, VEGF, and EMMPRIN . 5 × 10 5 A498, MCF-7 or U937 cells were incubated separately in a serum-free medium with or without TNFα (1 ng/ml) for 48 h, and with the addition of increasing amounts of recombinant EMMPRIN or the IgG Fc fragment (at 200 ng/ml). Concentrations of MMP-9 and VEGF were determined in the supernatants using ELISA ( n = 7).
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