Analysis of selected phospholipid species formed when A549 cells were treated with purified PA-LOX. A549 cells were incubated in the presence/absence of varying amounts of PA-LOX (50 μg, 150 μg, 500 μg/ml) for 12 h and 24 h. Lipid extracts were analyzed by reverse-phase LC/MS/MS, in negative mode, using Luna column on 6500 Q Trap. Single incubations were carried out for each PA-LOX concentration. Each sample was analyzed once by RP-HPLC and in triplicate LC-MS/MS.
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