FIGURE 5: deGFP standard curve at 4 μL volume with HBSS as diluent recorded on BMG Labtech PHERAstar FS plate reader (λEx = 485 nm, λEm = 520 nm). deGFP produced in myTXTL during this study was quantified according to the displayed equation, which resulted from linear regression fit. Each point was done in quadruplicate and the curve had an average percent CV of 4.05%.
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