Immunity to ΦNM1γ6 in liquid culture is unaffected by the presence of a tolerated chromosomal targetGrowth curves of the indicated chromosomal insertion strains from Fig. 3 harboring either spacer 43T or pGG3 CRISPR plasmids, in the absence (dotted lines) or presence (solid lines) of ΦNM1γ6 addition at a MOI of 10. Black arrow denotes the time of phage addition; no ATc induction is utilized in this assay. The presence of a chromosomal target for spacer 43T has no discernable effect on culture growth during spacer 43T-mediated immunity to ΦNM1γ6 (compare solid green and blue lines).
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