Effects of resveratrol and/or H 2 O 2 on the levels of TJ proteins. IPEC-J2 cells were pretreated with the indicated concentrations of RSV and then cocultured with 500 μ M H 2 O 2 for 4 hours. (a) The expression of claudin-1, occludin, and ZO-1 was detected by quantitative real-time PCR (qRT-PCR). Data are shown as ratios of abundance of target gene transcripts in the treated cells to those in the control cells after normalization to β -actin. (b) Protein levels of claudin-1, occludin, and ZO-1 were detected by Western blot with β -actin as the loading control. Values are the mean ± SE; n = 3. The columns with the same superscript capital letters and with different superscript capital letters mean no significant difference ( P > 0.05) and significant difference ( P
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