Tgfb1 promotes serotonin synthesis and kras V12 -induced carcinogenesis. Three-month-old adult zebrafish were treated for 7 days with 30 μg/mL dox with or without 2 μmol/L serotonin, BW23C86, PCPA, and SB204741. ( A ) IF staining of serotonin in liver section of these zebrafish. These slides also were co-stained for hepatocytes (HNF4a) and nuclei (4′,6-diamidino-2-phenylindole [DAPI]). Right : Quantification of serotonin-positive liver cells as percentages of hepatocytes (n = 10). Three-month-old Male kras V12 -expressing zebrafish were treated with dox with or without SB204741 and SB431542, respectively. ( B ) Gross morphology and H E staining of liver sections of these zebrafish. Quantification of tumor histology observed in the H E-stained liver sections of these zebrafish (n > 8 in each group). ( C–H ) IF staining and quantification of ( C ) PCNA+, ( D ) caspase 3+, ( E ) P-Erk+, ( F ) P-Smad2, ( G ) P-Tph+, and ( H ) serotonin+ cells in liver sections (n > 8 in each group). * P
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