Sre1 Controls Low Oxygen Expression of Multiple Tf2 Elements(A) Scheme for tagging individual Tf2 elements with ura4+. Bold arrow denotes Tf2 transcription initiation. Northern probe positions are indicated.(B) Yeast containing a single Tf2 element tagged by ura4+ or wild-type yeast were grown +/− oxygen for 8 h and processed for northern analysis using strand-specific DNA probes to detect either the tagged Tf2 element (ura4+ probe) or all 13 Tf2 elements (Tf2 probe).(C) Wild-type or sre1Δ yeast containing a tagged Tf2 element were grown +/− oxygen for 6 h and processed for northern analysis. All Tf2 elements tested (Tf2–4, Tf2–7, and Tf2–10) gave results identical to Tf2–3.
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