Section G: Gene manipulationYang Xu, College of Life Sciences Digestion: Restriction digests-II Reaction system: –All restriction enzymes require Mg 2
{"Image DocumentName": "Digestion: Restriction digests-II", "Image ImageDescription": "Reaction system: All restriction enzymes require Mg2+ (magnesium) usually at a concentration of up to 10 mM; but different enzymes require different : \uf0a7 pHs, \uf0a7 NaCl concentrations or \uf0a7 other solution constituents. Reaction process: The DNA is \uf0a7 incubated at the \uf0a7 optimum temperature (37\uf0b0C) with \uf0a7 the enzyme and \uf0a7 the appropriate buffer, in a volume of perhaps 20 \uf06dl. \uf0a7 A dye mixture is then added to solution, and \uf0a7 the sample is loaded on to an agarose gel. Section G: Gene manipulation. Yang Xu, College of Life Sciences.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}