"""Neuronal RIT1 expression increases HNPC proliferation and <t>Sox2</t> transcriptional activity. A and C , representative immunofluorescent images ( A ) and quantification ( C ) of the DG from DTG mice immunostained for <t>Nestin</t> ( green ) and Ki67 ( red ) to label proliferating neuronal stem cells. Nuclei (DAPI) are shown in blue. B , HNPCs were transfected with Myc-tagged RIT1 Q79L (CA) or EV, and proliferation was assessed by immunohistochemical detection of Nestin ( green ) and Ki67 ( red ) co-labeled cells. D , quantification of HNPC proliferation (Nestin + /Ki67 + ) as mean ± S.E. from three independent experiments. E , HNPCs were transfected as in A in the presence of a Sox2 luciferase reporter construct. Luciferase activity was evaluated 48 h post-transfection as described under """"Experimental Procedures."""" Results are presented as mean ± S.E. for three independent experiments repeated in triplicate. *, p"""
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