The SHP/RovS cell-to-cell communication system is active in strain NEM316. (A) Effects of culture media on shp expression. β-Galactosidase activity was measured in the WT-P shp strain grown in DMEMg, THY, or BHI medium at 37°C until it reached the early stationary phase (OD 600 , ~0.8). (B) Expression kinetics of the shp and rovS genes. β-Galactosidase activity was measured during the growth of the WT-P shp (○) and WT-P rovS (□) strains. Dotted lines indicate the growth of each strain as measured at an OD 600 , and solid lines indicate β-galactosidase activity. (C) shp expression depends on RovS and SHP. β-Galactosidase activity was measured in the WT-P shp , Δ rovS -P shp , and Δ shp -P shp strains grown in DMEMg at 37°C until they reached the early stationary phase (OD 600 , ~0.8). The data presented are the means ± standard deviations (SD) of results from three independent experiments. Differences were considered to be extremely significant if P values were
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