IL-26 suppressed the apoptosis of HSCs by inhibition of caspase 3 (CASP3) and Bcl-2 associated X protein (BAX). A. Apoptosis of HSCs by annexin V-FITC/PI double staining after treatment with IL-26 at 0, 50, 100, and 200 pg/ml for 48 h. B. The mRNA expression levels of CASP3 and BAX were detected by quantitative real-time PCR (qRT-PCR) in the different concentrations of IL-26 groups. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as the reference control. C and D. Western blotting analysis of the protein levels of CASP3, cleaved CASP3, and BAX in HSCs after IL-26 treatment. * P
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