Quantification of relative expression levels in four genotypes on suitable (pepper) and unfavorable (radish) hosts.The results represent the relative mRNA expression, with transcripts expressed by the aphids on pepper as calibrator and on radish as interest sample. Data were normalized for variation using GADPH expression. The green bars correspond to S genotypes (sensitive; N36-1 and Sur25A), the yellow bar corresponds to the SR genotype (simple resistant; Peralillo 1) and the red bar corresponds to the MR genotype (multiple resistant; 16A). Data represent mean ± SE of two different experiments, with three technical replicates each case.*p<0.05 and **p<0.01 indicate a significant difference compared to 1, used as a reference value for no change in expression using a t-test. Gene abbreviations: (A) cathepsin B-N, cathepsin B clade N; (B) HSP-70, heat shock protein 70; (C) GST, glutathione S-transferase; (D) Esterase E4/FE4, carboxylesterase type E or FE4; (E) CYP6CY3, cytochrome p450family CYP6CYP3; (F) CYP4, cytochrome p450 family CYP4.
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