Knockdown of <t>GRP78</t> sensitizes cancer cells to HDAC inhibitors-mediated apoptosis A , MDA-MB-435 cells were transfected with siRNA against GRP78 (siGrp78) or a random control (siCtrl), and 48 h later, were either non-treated (-) or treated (+) with TSA for 24 h. The upper panel shows GRP78 levels in the transfected cells with β-actin as loading control. Lower panel shows the percent of apoptotic cells under each condition as determined by mitochondrial membrane potential staining using the JC-1 assay. B , HCT116 cells were subjected to the same conditions as ( A ) and the cell lysates were assayed for <t>PARP</t> cleavage by Western blot. The normal length (116 kDa) and apoptotic, cleaved forms (85 kDa) of PARP are indicated. GAPDH was used as a control.
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