LG2809 and its RNA suppressed proliferation of CD4 + T cells stimulated with anti-CD3/CD28 treatment in the absence of antigen-presenting cells. Plates were first coated with 10 μg/ml anti-CD3ε monoclonal antibody (mAb) for 2 hr at 37°. Subsequently, splenic CD4 + T cells from BALB/c mice (5 × 10 4 cells/well) and 2 μg/ml anti-CD28 mAb were added with indicated doses of heat-killed LG2809 (a), LG2809 RNA (b) or RNA from murine splenic cells (c) and were cultured for 72 hr. The cells were pulsed with [ 3 H]thymidine for 24 hr, and [ 3 H]thymidine incorporation was measured. Data are shown as the mean ± SD. Data are representative of three independent experiments. Statistical differences were analysed using Tukey's tests. Data are statistically different ( P
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