Demonstration of <t>dsRNA</t> transmission from adult bee to Varroa via the bee hemolymph. Northern blot assay was performed on <t>RNA</t> extracted from a bee that had ingested dsRNA-GFP (B+) and from an untreated bee (B−), pooled RNA extracted from hemolymph collected from bees that had ingested dsRNA-GFP (H+) and from untreated bees (H−), and pooled RNA extracted from Varroa mites parasitizing dsRNA-GFP-treated bees (V+) and untreated bees (V−). C = positive control (GFP-carrying plasmid).
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