Metformin increased cell apoptosis as measured by p27, Bax, Bcl-2 and cleaved caspase-3 in MCF-7 cells. ( A ) Western blot of p27 and β-actin from MCF-7 cells that were treated with metformin (10 mM) for 24, 48 and 72 hours. ( B ) Western blot ratio analysis of p27 and β-actin. ( C ) Western blot of Bax, Bcl-2 and β-actin from MCF-7 cells that were treated with metformin (10 mM) for 24, 48 and 72 hours. ( D ) Western blot ratio analysis of Bax and β-actin, ( E ) Bcl-2 and β-actin, and ( F ) Bax and Bcl-2 at 24, 48 and 72 hours. ( G ) Western blot of cleaved caspase-3 and β-actin from MCF-7 cells that were treated with 10 mM metformin for 24, 48, and 72 hours. ( H ) Western blot ratio analysis of cleaved caspase-3 and β-actin at 24, 48, and 72 hours. * p
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