The activator of PGC-1α/peroxisome proliferator–activated receptor-α cascade, fenofibrate, attenuates LPS-induced ALI in mice. C57BL/6 mice (8 wk old) were instilled intratracheally with saline or LPS (2 mg/kg in 50 μl saline). At 4 hours after the intratracheal treatment, the mice were injected intraperitoneally with vehicle or fenofibrate (100 mg/kg body weight). At 24 hours after saline or LPS instillation, the mice were killed and BALF (0.5 ml for 3 times/mouse) collected. BALF was centrifuged to collect supernatants and cell pellets. Red blood cells in the cell pellets were lysed. Levels of indicated proinflammatory cytokines ( A ), protein concentrations ( B ), and total leukocyte numbers ( C ) in BALF was determined. ( A – C ) n = 3, 5, 3, 5 mice, respectively; mean ± SEM; * P
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