T reg 17 and T regDys alterations are accompanied by loss of IRAK-4 and mTOR activation in T regs in infected aged mice. Young and Aged mice were infected with CA or Sham as in Figure 8 . Flow cytometric analyses of CD3 + CD4 + gated cells for IL-17A and Foxp3 (A, B) , IFN-γ and Foxp3 (C, D) , on day 5 after infection. Statistical analyses of Foxp3 + IL-17A + (% T reg 17) (B) , and Foxp3 + IFN-γ + (% T regDys ) (D) , using data points from (A, B) , respectively. (E, F) Mice were infected for 2 days. Flow cytometry analyses of CD3 + CD4 + Foxp3 + gated cells for p-IRAK and IL-1R ( E , left and right) and p-mTOR ( F , top and bottom) in Foxp3 + T regs . Geometric mean fluorescence intensities (GM) are indicated in the histogram plots. Mean values ± SEM are plotted in statistical analyses. These data show one of four independent experiments showing similar results. (*P
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