"Phase contrast/fluorescence (A, D, J). phase-contrast (J), fluorescence micrographs (B, E. F, G, J) of isolated mitochondria (A), pt-division machineries (B), complexes of mt- and pt-division machineries (D, E, F), mt-division machinery (G), mitochondria and ZED (J), contraction model of pt-division machineries (C), an electron micrograph of the complex of isolated mt- and pt-division machineries (H), and SDS-PAGE (I). A. Physarum mitochondria with m-nuclei (bright rods) were drawn up in a ""Y"" image using optical tweezers. B. Manipulation of intact PD division machinery of C. merolae (a) and a dynamin-released PD division machinery (b) with the optical tweezer. The base line of one end of each of the pt-division machineries is fixed to the cover glass (yellow lines), while the other end is trapped by the optical tweezers (arrowheads) and an infrared laser (red lines). C. The schema shows a contraction model of pt-division machineries. In the first step, the dynamin molecules (red) drive the sliding of the fine filaments. In the second step, dynamin moves from the surface to the inside and pinches off the narrow bridge between daughter plastids. D. Mt-division machinery (yellow) is associated with pt-division machinery (red) in intact dividing cells. E and F. The bulk of isolated mt-division machinery (green) adheres to the pt-division machinery (red). G. Isolated mt-division machinery is a small ring. H. Immuno-electron micrograph showing the distributions of Mda1 and dynamin (Dnm2) in isolated mt- (large gold particles) and pt- (small gold particles) division machineries after negative staining. I. SDS-PAGE images of isolated PD machineries were compared with the interphase fraction (right). Proteins of isolated mitochondrial and plastid division machineries from cells in early M-phase (left), later M-phase (middle), and inter-phase (right) were separated by SDS-PAGE. J. In cells with transient DNA introduction and expression of ZED promoter without antisense-ZED, mitochondrial division (red) occurred normally (control). Plastid division occurred normally (phase contrast, PC), but mitochondrial division did not occur in ZED (blue)-downregulated cells (ZED antisense). Scale bars: 1 µm (A, B, G, J), 0.2 µm (H). B and C are from Ref. 28, D–J are from Ref. 30."
{}