Distribution of α-smooth muscle actin (αSMA) in paraffin sections from untreated (a,e,i,m), adjuvant control (b,f,j,n), low grade (c,g,k,o) and severe EAEO (d,h,l,p) testis at 30 (a–d), 50 (e–h) and 80 (i–l) days after the first immunisation. Panels m–p represent a higher magnification of images i–l. In the testis from untreated and adjuvant controls, αSMA is localised in the peritubular cells as a thin layer, but in low grade and severe EAEO testis, the αSMA is diffusely distributed within the cell. αSMA is also seen in the blood vessels (asterisks) within the testis. Scale bars represent 100 μm.
{}