D-isoAsp-H2B is enriched in active chromatin Chromatin was prepared from all 6 WT mouse brains (WT1-3 males, WT4–6 females) and each preparation was subjected to immunoprecipitations with antibody to acetylated histone H3 (H3K9; Ac) and with methylated histone H3 (H3K9Me3; Me). (A) The immunoprecipitates were than subjected to Western blotting using a pan-H3 antibody (H3), a pan-H2B antibody (H2B), and our D-isoAsp-H2B antibody (H2B*). (B) The Ac/Me band ratios are the same, as expected, when immunoblotting for pan-H3 or pan-H2B, whereas the ratio when using H2B* is about 55% higher.
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