SDS-PAGE and western blot analysis of gene expression in P . pastoris and transgenic maize. (A) SDS-PAGE analysis of recombinant Aga-F75 produced in P . pastoris . Lanes: 1, the purified Aga-F75 with PNGase F treatment; 2, the purified Aga-F75; M, the protein molecular markers. (B) Western blot analysis of Aga-F75M produced in transgenic maize. Lanes: 1, the protein extract of transgenic maize seeds with PNGase F treatment; 2, the protein extract of non-transgenic Zheng58 seeds as a negative control; M, the protein molecular markers; 3, the protein extract of transgenic maize seeds. (C) Specific expression of Aga-F75M in transgenic maize. Lanes: M, the protein molecular markers; 1, the purified recombinant Aga-F75 produced in P . pastoris ; 2, the protein extract of non-transgenic Zheng58 seeds; 3–5, the protein extracts of transgenic maize seeds; 6–8, the protein extracts of leaf, stem, and root of the transgenic plant, respectively.
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