RSR-2 is associated with chromatin and modifies RNAPII distribution.(A) Snapshot of the genome browser (chromosome IV) showing ChIP-Seq data. Chromatin-binding profiles of RNAPII and RSR-2 are similar. RNAPII peaks are represented in black, RSR-2 peaks are represented in red, and input samples are represented in blue. Upon rsr-2 RNAi, the RNAPII peak profile at phy-2 locus shifts from the 3′ to the 5′end. RSR-2 peaks disappear upon rsr-2 RNAi. Data was visualized with the Integrated Genome Browser (IGB) software. (B) ChIP-qPCR showing how RNAPII occupancy changes upon rsr-2 RNAi at the phy-2 locus. Black bars are a scaled representation of the regions covered by the primer pairs used in this experiment. (C) Distribution of RNAPII and RSR-2 ChIP peaks along five zones within an averaged gene. Statistically called peaks from a single ChIP-Seq experiment were classified with respect to their position relative to the nearest TSS. Y axis indicates the frequency of peaks within each zone. rsr-2 RNAi slightly modifies the distribution of RNAPII along an averaged gene.
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