Phosphorylation of eNOS by NRR volatile oil in cultured HUVEC cells.Cultured HUVECs were pre-treated with serum free medium or L-NAME (100 µM) for 3 hours, and treated with NRR volatile oil (25 µg/mL), VEGF (10 ng/mL, positive control) or control (serum free medium) for different time points (0 to 20 min). Phospho-eNOS Ser1177 (~135 kDa) and total eNOS (~135 kDa) were revealed by using specific antibodies (upper panel). Quantification of phospho-eNOS protein (at 5 min) from the blot was calculated by a densitometer (lower panel). Data were expressed as x Basal where the control (untreated culture) was set as 1, Mean ± SEM, n = 4, each with triplicate samples. **p<0.01.
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