Transient expression of chimeric antibodies carrying Nb5 in leaves of N . benthamiana . Protein extracts were analysed using (A) SDS-PAGE stained with Coomassie blue dye and (B) western blot developed with mouse anti-histidine tag monoclonal antibody. Extracts of N . benthamiana leaves transformed with Nb5-IgA and Nb5-IgY fusion constructs were tested. Leaves infiltrated with A . tumefaciens harbouring the vector pEAQ -HT were used as negative control.
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