FIGURE 8: Multi-variable titration of the T7p14-deGFP and P70a-T7rnap plasmids into 4 μL myTXTL reactions after 7.5 h of incubation. Readings were taken on a BMG Labtech PHERAstar FS (λEx = 485 nm, λEm = 520 nm). Each point was done in quadruplicate and the plot had an average percent CV of 14.51%. Peak production was found to be at 1250 pM T7p14-deGFP and 12 pM P70a-T7rnap with an average deGFP concentration of 18.95 nM.
{}