Interactions of mBPAG-C1 with aggregates of NF-L/ NF-M proteins. SW13.cl.2Vim− cells from transient transfections  of pcDNA-FLAG-mBPAG-C1 with (A and B) pRSVi-NFL1-415 and pRSVi-NFM; (C and D) pRSVi-NFL and pRSVi-NFM1-421; (E and F) pRSVi-NFL1-415 and pRSVi-NFM1-421 were  double-labeled with mouse anti-FLAG M2 mAb (A, C, and E)  and rabbit polyclonal antibody against NF-L (B, D, and F). A  normal filament network could be formed by wild-type NF-L and  tailless NF-M or by tailless NF-L and wild-type NF-M, but  mBPAG-C1 did not colocalize with these filament networks.  mBPAG-C1 only associated with the aggregates formed by tailless NF-L and tailless NF-M. Bar, 10 μm.
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