Characterization of four VAB-10–specific antibodies. (Top) Positions and names (Prot) of the peptides (horizontal double arrows; domain symbols are as in Fig. 2 A) used to raise antibodies (Ab), and of the epitope recognized by the mAb MH5 (vertical arrow). (Bottom) Western blots of total worm extracts probed with immunopurified antibodies (Ab) in the absence (−) or in the presence of a competing protein (C.P.). Competition with 4fr, kh2, and kh3 recombinant peptides were effective only with the cognate antibodies (e.g., 4fr did not compete interaction with mAb MH5).
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