GPAT1 was not required for the incorporation of exogenous fatty acid, and β-oxidation was higher in Gpat1−/− hepatocytes. Hepatocytes from wild type, Gpat1−/− (G1−/−), or Gpat4−/− (G4−/−) mice were labeled with 500 μM [1-14C]palmitic acid for 2 h. A–C, [1-14C]palmitic acid incorporation into triacylglycerol (A), phospholipids (B), and ASM (C). Data show means ± S.E. of two experiments performed in triplicate. Inset, comparison of cell media and intracellular (IntraC) ASM. Data show means of one experiment performed in quadruplicate. JBC(2013)288, 27299-27306
{}