Effect of pharmacological AMPK activation on insulin signaling in human myotubes. Myotubes were treated with 100 μmol/L A769662 and/or 100 nmol/L insulin for 30 min before (A) assaying 2-deoxyglucose uptake or 100 nmol/L insulin for 10 min before (B) immunoblotting to assess the phosphorylation status of PKB/Akt and ACC and the total protein abundance of PKB/Akt. Values are mean ± SEM from five separate experiments; glucose uptake values were performed in triplicate. *P < 0.05 vs. control cells.
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