A. The complete plasmid construct to be assembled with the GFP-tagged PIK3CA being inserted into the pcDNA 3.1/Zeo (+) backbone. gBlock pieces as well as the linear vector are shown in blue, with their overlapping region shown by a light green annotation labeling them as a ligation site. The PIK3CA gene is in yellow with the linker (purple) and GFP tag (green) also annotated. Other important components of the plasmid machinery are labeled as necessary.
{}