"""m 6 A depletion induces exon exclusion in synapse-associated genes. (A) Genes producing exon-excluded transcripts in P7 cKO cerebellums as compared with the Ctrl. (B) Enriched pathways of genes producing exon-excluded transcripts in P7 cKO cerebellums as compared with the Ctrl. Enriched pathways under the """"Neuronal system"""" are expanded on the right. (C) Production of exon-excluded transcripts of major synapse-associated genes in P7 cKO cerebellums. Left, IGV tracks displaying the RNA-seq reads coverage in P7 Ctrl (blue) and cKO (red) cerebellum, the excluded exons are shaded with pink columns. Middle, calculated exon-exclusion percentage in P7 cKO cerebellums by the PSI value. Right, semiquantitative PCR detection of exon-excluded transcripts in P7 cKO cerebellums. (D) Images of the CGCs in the calcium detection assay. Grin1 001 or Grin1 011 was introduced into the cKO CGCs by electroporation and was co-expressed with <t>mCherry</t> (red). Fluo-4 (rainbow) indicated the cellular calcium concentration in the CGCs. Scale bar, 5 μm. (E) Statistical analysis of the cellular calcium concentration in the calcium detection assay. (F) CCK8 assay shows the survival rate of the Ctrl CGCs with overexpression of mCherry and cKO CGCs with overexpression of Grin1 001 or Grin1 011 in 3 DIV and 5 DIV. (G) Schematic diagram illustrating the mechanisms underlying the regulatory functions of METTL3-mediated m 6 A modification in cerebellar development. Red dots represent m 6 A modification. The box and line symbols in the right part of the diagram represent pre-spliced RNAs, with exons shown as boxes and introns shown as black lines. Exons in post-spliced RNAs are connected by red lines. Further information about this figure can be found in S1 Data . The data were represented as means ± SEM. For calcium concentration detection, n = 10; for CCK8 assay, n = 3. * p -value"""
{}