Flow cytometry analysis of P-selectin expression on  platelets. Wild-type and ΔCT platelets were stained for membrane P-selectin and analyzed by flow cytometry. In the resting  state, platelets of both genotypes displayed virtually no P-selectin  on their plasma membranes. Thrombin activation induced in  wild-type as well as in mutant platelets a similar increase in mean  fluorescence with ∼90% of P-selectin–positive platelets. Representative histograms are shown. Shaded area, negative control  staining with only the FITC-conjugated goat anti–rabbit IgG.
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