Autoinhibition of Jak2 in E. coli . Gst-fusion proteins for JH1 (JH1-Gst) and JH1–2 (JH1–2-Gst) domains of Jak2 were expressed in bacterial cells as described in MATERIALS AND METHODS. The cells were lysed by boiling in reducing Laemmli sample buffer. Lysates were separated in 7.5% SDS-PAGE, and analyzed in anti-HA (right) and anti-phosphotyrosine (left) immunoblots. Arrows indicate the migration of JH1-Gst and JH1–2-Gst. The mobilities of the molecular mass markers (in kilodaltons) are shown on the right.
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