miR-199a-3p regulated RA-FLS proliferation and apoptosis partially via suppressing RB1 RA-FLS was transfected with an miR-199a-3p-expressing plasmid (miR-199a-3p) or a pSliencer 4.1-CMV puro vector (miR-control) or miR-199a-3p in combination with an RB1-overexpression plasmid (miR + pcDNA-RB1) or miR-199a-3p in combination with a negative control plasmid (miR + pcDNA-NC). ( A ) Western blot of RB1 protein in RA-FLSs transfected with negative control (NC) or pcDNA3-RB1. ( B ) Proliferation of transfected RA-FLS was measured by the MTT assay. ( C ) Apoptosis was measured by FITC Annexin V and PI staining followed by flow cytometry. ( D ) Caspase-3 activity was measured by a colorimetric method. n =6; * P
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