TNF-α induces PPP2R2B methylation, abolishes B55β expression, and impairs CWID in healthy T cells. ( A ) T cells from HDs were activated and expanded in the presence of IL-2 for 10 days. In addition to IL-2, at days 0, 2, 4, 6, and 8, the indicated cytokines (TNF-α, IFN-α, IL-6, IL-21, or IL-17) were added to the culture. At day 10, cells were counted, washed, and replated in the absence of IL-2 and proinflammatory cytokines. ( B ) Apoptosis (annexin V + SYTOX Orange − ) was quantified before (0 hours) and after IL-2 withdrawal ( n = 3–6). * P
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