Interactions of <t>human</t> bone marrow-derived MSCs and <t>prostate</t> cancer <t>cells.</t> (A) Migratory response of human MSCs to the medium, <t>normal</t> prostatic <t>epithelial</t> cells (PrEC) and prostate cancer cell lines (DU145, PC3 and LNCaP). Migrated cells through the membrane of transwells were stained with crystal violet 8 h after cell plating. The lower surface of the membrane was photographed (100x), and a representative image from each condition is shown at top. Quantitative data are represented as the means ± SD of the number of cells per high-power field (100x) in triplicate experiments. (B) Macroscopic (upper) and microscopic (bottom) view of cellular aggregates under 3-D RWV culture conditions of 3A6 cells alone (3A6/3A6) or mixed with LNCaP (3A6/LNCaP), C4-2 (3A6/C4-2) or PC3 (3A6/PC3) cells. (C) Detection of mixed cellular components in the 3-D cultured prostate tumoroids by H E and immunohistochemical staining of E-cadherin (E-Cad) in the cell aggregate sections.
{}