Overexpression of EGFR axis signaling components reverses the loss of HNSCC cell viability mediated by miR-27a*, which increases apoptosis and reduces migration(A) The effect of miR-27a* on cell viability is decreased in 22A and 22B cells by overexpression of EGFR, AKT1 and mTOR 48 hrs prior to miR-27a* expression as compared to control vector, *p<0.01; (B) Overexpression following transfection with EGFR, AKT1 and mTOR (EAmT) vectors was confirmed by immunoblot analysis. (C) Annexin V assay shows increased apoptosis in HNSCC cells after transfection with miR-27a*, *p<0.005; (D) Transfection with miR-27a* leads to increased PARP cleavage compared to miR-27a and -Control; (E) Wound healing assay demonstrates decreased migration distance after 24 hrs after transfection of miR-27a* compared to miR-Control, −27a, and −7, *p<0.001.
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