Time course of activation of pro-enzymes to the mature and active form of the wild-type and the mutants of Erv-C.Aliquots of purified pro-enzymes (10–20 µg) were treated for activation for 0 to 50 minutes to convert into their respective mature forms and the percentage of residual enzyme activities were determined with respect to the maximum activity using an azocasein assay, as described in Materials and methods.
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