Glucose supplementation rescues mitochondrial mass and increases oxygen consumption and ATP levels in G3 mTerc−/− mice.12–15 month old G3 mTerc−/− mice with weight loss on normal ad libitum diet and age-matched mTerc+/+ mice were analyzed under continuous exposure to normal ad libitum diet or 2 weeks after switching to a glucose enriched diet (+Glc): (a–d,g,f) Histograms showing (a) the mitochondrial DNA (mtDNA) copy number in the indicated tissues, (b) the expression of PGC-1α and PGC-1β in the indicated tissues, (c) hepatic citrate synthase (CS) expression, (d) hepatic cytochrome c oxidase levels (COX), (f) oxygen consumption of freshly isolated haemtopoietic cells (Lin-negative), and (g) ATP-levels of freshly isolated haemtopoietic cells (Lin-negative). Data in all histograms are shown as relative expression levels/numbers with data for mTerc+/+ mice on normal ad libitum diet being set to 1. (a-d,g) n=4–6 mice per group, (f) n=9–17 mice per group. (e) Representative western blot analysis of OXPHOS enzymes ((NDUFA (CI), 30 kDa su (CII), ATPα (CV), Core 2 (CIII)) in liver homogenates. All statistical data were assessed using Student's t-test and are presented as mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001.
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