The effect of butanol fraction of A. indicum to induce mRNA expression of the PPARγ target gene. 3T3-L1 preadipocyte cells were differentiated. Twenty-four hours before and during differentiation, cells were treated with vehicle control (DMSO) or 100 μg ml−1 of butanol fraction. mRNA expression of PPARγ, LPL, aP2, adiponectin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were measured at Days 0, 2, 4, 6 and 8 of differentiation.
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