Western blotting is carried out as such
{"Image DocumentName": "Western blotting is carried out as such", "Image ImageDescription": "Proteins are extracted from the cell(s) and purified. They are then separated by SDS-PAGE. They are first dissolved in the detergent sodium dodecyl sulfate. This denatures proteins and coats them with negative charges. The negatively charged proteins are then separated by polyacrylamide gel electrophoresis. They are then blotted onto nitrocellulose or nylon filters. The filters are placed into a solution containing a primary antibody (recognizes the protein of interest) A secondary antibody, which recognizes the constant region of the primary antibody, is then added. The secondary antibody is also conjugated to alkaline phosphatase. The colorless dye XP is added. Alkaline phosphatase converts the dye to a black compound. Thus proteins of interest are indicated by dark bands", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}