Reverse-transcription-polymerase chain reaction (RT-PCR) analyses of stress-related genes in TheIF1A-transformed tobacco plants. (A) Gel electrophoresis of tobacco GST, MnSOD, poxN1, NtMPK9, CDPK15, and TOBLTP. Tubulin was used as the reference gene. (B) Relative expression level according to tubulin based on (A). All experiments were repeated three times. Data represent the means ± SD of three independent experiments. ∗ Indicates significant differences between the transgenic lines and WT (p < 0.05).
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