IαI and IαI domain HC2 can support attachment and survival of hPS cells. ( a ) Quantification of cell attachment by crystal violet staining 4 h after seeding of hiPS cell line K2C added to plates with 10 μg ml −1 VN-XF coating, 50 μg ml −1 IαI medium supplementation, 50 μg ml −1 HC1 coating or 50 μg ml −1 HC2 coating. ( b ) Immunofluorescence of hES cell line HUES1 on VN-XF coating or IαI supplement showing E-cadherin (left panel) and Oct4 (right panel), scale bar shows 100 μm. ( c ) Cell survival and growth assayed with crystal violet staining after 4 days of culture of HUES1 and K2C in E8 medium with IαI and different blocking antibodies, ( d ) E8 medium with or without 50 μg ml −1 IαI (E8:IαI), 5% BSA (w/v) in E8: IαI, and mTeSR1 medium with or without IαI; ( e ) E8 medium supplemented with IαI and IαI together with different integrin-blocking peptides. All cell-number quantification experiments were performed in triplicate over three separate experiments. Bars show mean±s.e.m. and statistical analysis over the three independent experiments. Statistical analysis indicates significant differences with * P
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