Effects of AAPH on yolk-sac blood vessels of chick embryo.(A) Representative appearance of yolk-sac blood vessels of HH 18 embryo treated with saline, (B) 4 µmol AAPH and (C) 5 µmol AAPH for 12 hours. The silastic rings in A–C had inside diameter at 9 mm and outside diameter at 11 mm. The yolk-sac blood vessel images were taken by a stereomicroscope (Olympus MVX10 with OPTPRO 2007 image acquisition system) with resolution ratio at 1024×768 (Scale bar = 1 mm). (D) Statistical chart showing the yolk-sac blood vessel density from AAPH treated and untreated embryos. The results represent the mean ± S.D (n = 10). Statistical significances were determined using SPSS13.5 software, **p<0.01 compared with control group.
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