Western blot of recombinant prion protein (PrP) 5 ng (lane 1), CHO cells (lanes 2–5) and Vero cell (lanes 6–9). Cells exposed to normal bovine brain and passaged 30 times (lanes 2, 3, 6, 7). Cells exposed to bovine spongiform encephalopathy agent and passaged 30 times (lanes 4, 5, 8, 9). Total PrP (cell extracts without proteinase K [PK] digestion) are shown in lanes 2, 4, 6, 8; cell extracts treated with PK are shown in lanes 3, 5, 7, 9. Western blots were probed with PrP monoclonal antibody 6D
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