Allowing annealing of the primers to the single-stranded DNA template.
{"Image DocumentName": "Allowing annealing of the primers to the single-stranded DNA template.", "Image ImageDescription": "Annealing step. The temperature is lowered to 50\u201365 \u00b0C for 20\u201340 seconds. Allowing annealing of the primers to the single-stranded DNA template. This temperature needs to be low enough to allow for hybridization of the. primer to the strand, but high enough in order for the hybridization to be. specific. TWO.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}