The induction of HK2 expression in HK2negative SNU449 cells by treatment with 5-Aza-CdR and hypoxia ( A ) Hypoxia-mediated HK2 expression. ( B ) The suppression of HK2 expression by HIF-1α silencing. ( C ) The methylation status of the HK2 promoter CpGs plotted for the 5-Aza-CdR-treated SNU475 cells and SNU449 cells. The difference in methylation frequency between 5-Aza-CdR-treated cells and untreated cells is shown in each lower panel. ( D ) The induction of HK2 expression in SNU475 and SNU449 cells by treatment with 5-Aza-CdR for 2 d, followed by hypoxic stimuli for 1 d. In all experiments, the expression of HIF-1α and HK2 were evaluated by immunoblot. ( E ) The interaction between the −234/−230 HRE and HIF-1α following 5-Aza-CdR treatment was evaluated using a ChIP assay.
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