Cloning Process (Page 2)
{"Image DocumentName": "Cloning Process (Page 2)", "Image ImageDescription": "5 steps (3-4) 3. Combine the 2 DNA pieces (into a recombinant plasmid?) Recombinant plasmid \u2013 plasmid + DNA fragments. Sealed together using DNA Ligase. Remember: we used ________ ________ to cut gene of interest from original site & cut vector\u2019s DNA. This ensures matching sticky ends on gene of interest & vector DNA. 4. Transfer the vector (recombinant plasmid) into a host cell. Usually involves bacterial transformation.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}