Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, D3 mouse embryonic stem cell line, and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM3/Fragilis at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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