Shikimate and phenylalanine/tyrosine biosynthesis in Kluyeromyces marxianus . The genes are named according to their orthologues in Saccharomyces cerevisiae . Genes in red are deleted or otherwise inactivated, genes in green are overexpressed in our study, and genes in blue are overexpressed alleles of genes resistant to feedback inhibition. Red dashed lines indicate product inhibition, while dashed arrows indicated multiple reactions between the metabolites indicated. Compounds that were assayed by HPLC are italicised. Abbreviations: PEP: phosphoenolpyruvate, E4P: erythrose 4-phosphate, DAHP: 3-deoxy-D-arabinoheptulosonate-7-phosphate, DHQ: 3-dehydroquinate, 3-DHS: 3-dehydroshikimate, SHIK: shikimic acid, S3P: shikimate-3-phosphate EPSP: 5-enoylpyruvateshikimate-3-phosphate, CHOR: chorismate, PPH; prephentate, (H)PPY: (hydroxyl)phenylpyruvate, 2-PE: 2-phenylethanol; PAA: phenylacetic acid; TYROSOL: tyrosol/para-hydroxyphenylethanol; PHPA: para-hydroxphenylacetate; TRP: tryptophan; TYR: tyrosine, PHE: phenylalanine.
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