Incorporation of the G-quadruplex motif and not sequence per se induces promoter activity. (A) Scheme showing the constructs made to insert either a G-quadruplex-forming (G4) or disrupted G4 (disG4) as control sequence upstream of SV40 promoter in a luciferase reporter vector. (B) CD spectra of oligonucleotide used for G4 motif and disG4 showing disruption of the quadruplex motif in case of disG4. (C) Luciferase reporter activity of clones harbouring G4 or disG4 in human cell lines with respect to the no-insert construct. All experiments were done in triplicate using Renilla luciferase activity as transfection control.
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