SNC1-RLD encodes a truncated TNL protein. (A) Alignment of deduced amino acid sequences of SNC1-Col (top) and SNC1-RLD (bottom) using the EBI-ClustalW tool ( http://www.ebi.ac.uk/Tools/clustalw/ ) [54] . Identical amino acids are indicated by asterisks. Colons and semi-colons show conserved substitutions and semi-conserved substitutions, respectively. Characters in blue, red and <t>green</t> show the amino acids corresponding to exon 1, exon 2 and exon 3, respectively. (B) SNC1 gene model as experimentally verified by reverse transcription <t>PCR</t> and 3′-RACE from Col-0 (middle) and RLD (bottom) compared with the TAIR9 gene model (top). Exons are indicated by boxes, introns by lines, and stop codons by red asterisks.
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