"""Capsular α-glucan production of P i -stressed M. smegmatis , M. marinum , and auxotrophic M. tuberculosis . The mycobacterial strains M. smegmatis mc 2 155, M. marinum E11, and auxotrophic M. tuberculosis mc 2 6020 were grown in 7H9-based liquid medium supplemented with different concentrations of P i , and cells were collected at various time points (see below) The bacterial cells were """"shaved"""" with a detergent, and the obtained capsular extracts were subsequently assayed by immunodot-blotting to analyze the capsular α-glucan level using anti-glucan mAb (IV56B6). A , growth curves of M. smegmatis grown in different concentrations of P i show a P i concentration-dependent growth arrest. B , immunodot-blotting analysis of capsular α-glucan of A at 48 h revealed P i stress-mediated induction of α-glucan in M. smegmatis. C , immunodot-blotting analysis of capsular α-glucan in M. marinum ( left panel ) and M. tuberculosis ( right panel ) grown under low and high P i at days 5 and 6, respectively. In both mycobacterial strains, the capsular α-glucan levels are elevated under low P i . The data presented here are representative for four independent experiments. Dil , diluted/dilutes."""
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