The loss-of-function in individual members of the carotenoid cleavage dioxygenase gene family cannot restore plastid development in ccr2 rosettes. Three-week-old WT, ccr2 , ccr2 ccd1, ccr2 ccd4, ccr2 ccd7 , and ccr2 ccd8 (F 3 homozygous double mutant lines) plants were shifted from a 16-h to 8-h photoperiod until newly formed leaves in the ccr2 rosette displayed a virescent leaf phenotype. (A) Representative images of plants showing newly developed leaves in the rosette. (B) Quantification of leaf variegation in individual rosettes from ccr2 ccd double mutants. Data is representative of multiple independent experiments. Statistical analysis by ANOVA with post-hoc Tukey test showed no significant difference in the number of ccr2 and ccr2 ccd plants displaying a virescent phenotype.
{}