Mutation of the esx-5 RegX3 binding site suppresses overexpression of esx-5 genes and hypersecretion of EsxN by the Δ pstA1 mutant. (A and B) Transcript abundances of pe19 , espG 5 , eccD 5 , udgA , and mgtA relative to sigA were determined by quantitative RT-PCR for the indicated strains grown to mid-logarithmic phase in 7H9 complete medium. Results are the means ± standard deviations for three biological replicates, each run in technical duplicate. **, P
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