Expression in Standard and Gateway®-Modified Vectors
{"Image DocumentName": "Expression in Standard and Gateway\u00ae-Modified Vectors", "Image ImageDescription": "GUS. 6xHis-Gus. GST-GUS. Trx-GUS. U. I. Std. GW. MW. 50 kDa. E. coli strain BL21-SI. U = Uninduced, I = Induced. The GUS gene was cloned using restriction enzymes and ligase (Std) into expression vectors or transferred via Gateway\u00ae Technology into destination vectors for native or fusion protein expression (GW). Expression clones generated via Gateway\u00ae Technology are flanked by attB sites. Induced cultures of BL21-SI\u2122 containing these constructs show similar levels of protein expression suggesting the attB sites have no detectable effect on protein expression levels. What is the effect of Gateway\u00ae cloning on protein activity? We subcloned the GUS reporter gene into a mammalian expression vector, transfected cells, and then stained for activity.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}