Nested PCR-RFLP (restriction fragment length polymorphism) of SSU rRNA gene for the identification of E . histolytica and E. dispar . Lane 1 , molecular weight marker; lanes 2–5 , Dra I digested PCR products; lane 6 , DNA positive control for E. histolytica (c+eh); lane 7–10 , Sau 96I digested PCR products; lane 11 , DNA positive control for E. dispar (c+ed); and lane 12 , negative control.
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