Anti- TRPM8 siRNA up-regulates expression of p21 CDKN1A and p27 CDKN1B . Total RNA extracted from PANC-1 and BxPC-3 cells 72 h following transfection with siRNA directed against TR PM8 or non-targeting control siRNA was analyzed for p21 CDKN1A and p27 CDKN1B mRNA levels by quantitative real-time PCR. Each value represents the relative p21 CDKN1A or p27 CDKN1B mRNA level in the anti- TRPM8 siRNA transfected cells as compared to that in control siRNA transfected cells. This experiment was independently performed twice, and the same pattern of changes in p21 CDKN1A and p27 CDKN1B mRNA was observed.
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