Knockdown of OPA3 induced changes in cell morphology and sensitized cells to F-actin rearrangement induced by TGF-β.(A and B) Effects of Smad2 on changes in cell morphology and OPA3 level by TGF-β treatment. (B) APRE-19 cells were transfected with Smad2 siRNA or control siRNA. Forty-eight hours after transfection, cells were incubated in the absence or presence of TGF-β for 48 h. Cells were analyzed with phase contrast microscopy (A). Real-time PCR evaluated the level of OPA3 mRNA (B). (C–G) Effects of OPA3 depletion on cell morphology and F-actin rearrangement. APRE-19 cells were transfected with OPA3 siRNA or control siRNA. Forty-eight hours after transfection, cells were treated with TGF-β for the indicated periods of time. Cells were analyzed by Western blotting (C) and Real-time PCR (D). Cells were fixed and stained with phalloidin-TRITC (E). TRITC intensity (F) and Cell lengths (G) were analyzed using confocal images. Data are the mean ± SD of three experiments, each with >50 cells per condition. *P<0.05; **P<0.005.
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