TRF2 binds to telomeric DNA (DNA) and nucleosomal array fibers (NA). TRF2 ( A ) or TRF2 BH ( B ) binding to substrates detected by electrophoresis on 0.3% agarose gels or 0.6% agarose gels to detect binding of TRF2 B ( C ). DNA and nucleosomal arrays pertain to pRST5 digested to obtain a 2 kb fragment containing the 580 bp telomeric DNA (Tel) with a 1 kb and smaller fragments being non-telomeric (NT). 0.6% agarose gel to detect binding of TRF2 to nucleosomal arrays derived from digestion of with SfaNI/PvuII/BspHI ( D ).The 0.3% agarose lanes in (A) and (B) were formed using a multi-gel apparatus as described in Materials and Methods . Red arrows point to mobility shifts produced by TRF2 or TRF2 BH complexes.
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