Pharmacological blockade of FASN activity suppresses the HER2-induced transforming phenotype in breast epithelial cells Left. Transcriptional activities were expressed as relative (×-fold) changes in luciferase activity of FASN promoter-transfected MCF10A and MCF10/HER2 cells in response to treatments after normalization to pRL-CMV luciferase activity. Each experimental value represents the mean fold increase ( columns ) ± SD ( bars ) from at least three independent experiments measured in triplicate. Right. Soft-agar colony formation assay of MCF10A and MCF10A/HER2 cells cultured with/without C75. Each experimental value represents the mean colony number ( columns ) ± S. D. ( bars ) from at least three independent experiments measured in triplicate.
{}