miR-584-5p promoted cellular senescence and negatively regulated TGFβ signaling pathway via downregulation of WWP1. a . miR-584-5p up-regulation or WWP1-shRNA promoted cellular senescence by detecting SA-β-gal activity. The rescue experiments for miR-584-5p overexpression were performed by ectopic expression of WWP1 without its 3'-UTR in MGC803 cells. b . Similar rescue experiments for miR-584-5p silencing was performed by down-regulation of WWP1 in SGC7901 cells. c . The expression levels of senescence-related proteins were evaluated by Western blot. d . Expression of p53 signifcantly increased by miR-584-5p was ameliorated by the p53 inhibitor pifthrin-α. e . Treatment of pifthrin-α signifcantly receded miR-584-5p induced apoptosis of the MGC803 cells which was measured in a flow cytometer. f . Positive SA-β-Gal stained cells induced by miR-584-5p was confined by treatment of pifthrin-α. h . Levels of key proteins involved in TGFβ signaling by western blot. i . Levels of key proteins involved in TGFβ signaling in MGC803 cells treated with LY364947 were measured by western blot. j . The expression level of TGFβ was determined in MGC803 and SGC7901 by immunofluorescence assays. Scale bar is 25 μm. * p
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