Absence of TERT in ctc1 mutant plants leads to telomere shortening and increased cytogenetic damage. (A) Phenotypes of the mutants six weeks after germination. (B) Images of flower pistil mitotic anaphases showing chromosome bridges with subtelomeric signal in ctc1 tert analysed by FISH with the nine subtelomeric BAC fluorescent probes (magenta). DNA is stained with DAPI (blue). Bar = 2 μm. (C) Percentages of anaphases with chromosomal bridges and the percentages of anaphases with subtelomeric signal in bridges (from three different plants in each case) of ctc1 tert. (D) TRF analysis of bulk telomere length in DNA from flower buds of CTC1 TERT, CTC1 tert, ctc1 TERT and ctc1 tert mutants using telomeric (left) the chromosome 2 subtelomeric (right) probes.
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