MCF7 and HeLa cell proliferation rates were assessed at baseline, 24 hours, 48 hours, 72 hours, and 96 hours following transfection with a TIMELESS siRNA and a scrambled sequence negative control oligo. (A) Transfection with TIMELESS siRNA in MCF7 cells slowed down cell proliferation compared to negative controls (P < 0.05); (B)TIMELESS knockdown did not result in a significant reduction in cell proliferation rate in HeLa cells. Error bars represent standard deviations.
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