RUNX1 was a direct target of miR-296-5p in CRC cells. ( A ) The potential binding sites of RUNX1 and miR-296-5p were predicted by starBase. ( B ) The luciferase activity was tested in HCT116 and SW620 cells co-transfected with RUNX1 3ʹUTR-WT or RUNX1 3ʹUTR-MUT and miR-296-5p or miR-NC. ( C and D ) QRT-PCR and Western blot analyses were conducted to determine the mRNA and protein expression of RUNX1 in HCT116 and SW620 cells transfected with miR-296-5p or miR-NC. ( E ) The abundance of miR-296-5p was measured by qRT-PCR in HCT116 and SW620 cells transfected with anti-NC or anti-miR-296-5p. ( F and G ) The mRNA and protein levels of RUNX1 were measured in HCT116 and SW620 cells transfected with sh-NC, sh-circ#2, sh-circ#2 + anti-NC, or sh-circ#2 + anti-miR-296-5p. * P
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