PA0833 stimulates protective immunity in a P. aeruginosa sepsis model. (A) Comparison of total <t>IgG</t> and IgG subgroups <t>(IgG1,</t> <t>IgG2a,</t> and <t>IgG2b)</t> in the mice ( n = 5) immunized with PA0833. Serum was obtained at 7 days after the final immunization, and the levels of total IgG and IgG subgroups were expressed as the means of log 2 titers. Multiple comparisons among different groups were analyzed using one-way ANOVA. Data are shown as the means ± SD. (B) BALB/c mice ( n = 10) were immunized with PA0833 plus an Al(OH) 3 adjuvant and challenged with PAO1 at 7.0 × 10 7 CFUs/mouse by intravenous injection. The survival rate was monitored for 14 days. The P -values were calculated using the Mantel–Cox log-rank test. (C,D) Efficacy of immunization with PA0833 on the spread of P. aeruginosa . The number of viable bacteria in the blood, liver, and spleen of mice ( n = 10) at 1 and 3 days post-infection are shown. Data are presented in box and whisker plots, and the medians are shown. Differences were compared to determine their significance using Student's t -test.
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