Proposed model for interaction of Pub1 and Fur4.The uracil transporter Fur4 localizes to Golgi or vacuoles under normal growth conditions and shifts to the plasma membrane when nitrogen is consumed. Fur4 is also localized to the plasma membrane when the pub1 gene is deleted. Increased uracil uptake in pub1 mutants by the accumulated Fur4 localization in the membrane rescues the cell lysis phenotype. Ubiquitination of Fur4 is observed, but the mechanisms underlying Pub1 regulation of Fur4 localization remains to be determined. PM, Plasma Membrane.
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