Cytosolic microinjection of cytochrome c induces death in fibroblasts but not cardiomyocytes. Neonatal rat cardiomyocytes and dermal fibroblasts were microinjected with 25 μg/μl of bovine or yeast cytochrome c , and cell survival (using morphological criteria) was assessed at multiple times after the injections. (A) Phase-contrast and fluorescence photographs of the cells 3 h after the injections of cytochrome c . The injected cells (arrows) were identified by the presence of rhodamine dextran coinjected with the cytochrome c . (B) Quantitation of cell survival. Data shown are the mean ± SEM of three independent experiments.
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