Expression of DPT in epidermis and dermis.(a) Agarose gel images showing DPT PCR products, 168 bp. (i–v) and RPL32 PCR products, 147 bp. (vii–xi). Lanes: i & xi) No template control for DPT and RPL32 respectively, ii & x) NSF, iii & ix) HaCaT cells, iv & viii) Epidermis, v & vii) Dermis, vi) 100 bp DNA ladder. (b) Western blotting analysis of protein homogenates from i) HaCaT cells and ii) epidermis revealing the absence of DPT protein in contrary to the prominent expression in v) dermal and vi) NSF protein extracts. The bands were compared with iv) rDPT and iii) protein molecular weight marker to determine the band size.
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