Omp19 expression in plants.(A) SDS PAGE of different fractions obtained from immobilized metal affinity chromatography. 1, Marker; 2, crude extract; 3, flow through; 4, first wash; 5, second wash; 6, eluate. Marker band sizes are indicated on the left in kDa. (B) Immunoblot of plant extracts. Crude leave extracts were separated in a 12% SDS-PAGE, transferred to a PVDF membrane and probed with Omp19 antisera. 1: WT; 2,3,4: Crude protein extracts of leaf material corresponding to 50 µg, 25 µg, and 12.5 µg of total protein, respectively. 5: 1 µg purified Omp19.
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