Levels of CD4+IFN-γ+ T cells induced by ex vivo stimulation with recombinant Ag85, MPT51, and HspX.Thirty days after vaccination, lung and spleen suspensions were stimulated exvivo with Ag85, MPT51, HspX, or medium alone. The number of cells positive for CD4 and IFN-γ was determined by flow cytometry. Lymphocytes were selected based on size and granularity. Gates were set to analyze CD4+ T cells, and then the fluorescence of antibodies detecting IFN-γ+ cells was recorded. (A–B) Spleen cells from mice vaccinated with (A) rBCG-CMX or (B) BCG. (C–D) Lung cells from mice vaccinated with (C) rBCG-CMX or (D) BCG. In A and C, all results were different from the medium stimulation. These data are representative of two independent experiments (N = 6, *p<0.05).
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